Biological, Serological and Molecular Characterization of Tomato yellow leaf curl virus

Abstract

Experiments were carried out during the growing season of 2014-2015 in the laboratory of Plant Virology and greenhouses, Plant Protection, Faculty of Agriculture, Karbala Univ.Whitefly-mediated inoculation of some indicator plants (Solanum lycopersicon , Datura stramonium, Nicotiana benthamiam , N. tabacum cv. White Burley, N. tabacum cv. Samsun ,N. glutinosa, Phaseolus vulgaris ,Abelmoschus esculentus, Gossypium hirsutu, Physalis floridana ,Chenopodium amaranticolor, Cucurbita pepo, Cucumis sativus, Cucumis melo ,Solanum melongena) have been employed to identify the virus under study. Tomato plants infected with virus have shown symptoms of leaf wrinkling and yellowing with the stunting of plants and dropping of flowers. Datora plants have exhibited symptoms associated with the TYLCV that its presence has been confirmed by ELISA and PCR. Inoculation of N. glutinosa, N. tabacum var. Samsun and N. benthamiam, Beans and Okra have not produced any kind of symptoms and the presence of the virus has been detected by ELISA test and PCR. ELISA and PCR-based results have proven that plants of N. tabacum var. While Burly, Cotton, Ground cherry, Goosefoots, Squash, Cucumbers, Eggplant and Watermelon are immune to TYLCV. From the Nucleotide sequence analysis of different isolates of TYLCV collected from some provinces Muthanna, new strains of TYLCV have been recorded in the National Center for Biotechnology Information (NCBl). The isolate A4MA obtained from the desert region farms in Muthanna province have shown 99% similarity to the nearest neighbor TYLCV (JQ354991.1).

Malik H. Karem1, Agric. College, AlMuthanna Univ.

     Aqeel N. Barbar Agric.College, Karbala Univ.

Falah H. Issa1, Agric. College, AlMuthanna Univ

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