Effect of cryopreservation on some semen parameters of Awassi rams

Mohamad A.Ishak⃰ , Ali.A. Al-sadoon⃰⃰ ⃰ ⃰⃰⃰, Mohammed- B.M-R. Fakhrildin⃰
⃰ University of Baghdad / college of Agriculture,⃰ ⃰⃰⃰ University of al-Nahrain / High Institute of Infertility Diagnosis and Assisted Reproductive Technology,
⃰⃰⃰ ⃰ ⃰ University of Al-Muthanna / college of Agriculture


Volume 4, Issue 1, 2016 Page 133-140

Abstract

This experiment was conducted at the station of sheep and goat research’s / the state board of agricultural research ,the artificial Insemination Department belongs to the Ministry of  Agriculture and in the High Institute of Infertility Diagnosis and Assisted Reproductive Technology at Al-Nahrain university, Baghdad, Iraq to investigate the effects of  cryopreservation on some ram semen characteristics. Six Turkish Awassi rams were used for semen collection and evaluation. several samples of ram semen were collected and evaluated for Volume, pH,color,Sperm concentration ,Mass activity and individual motility using computer assisted semen analysis (CASA) Dead and abnormal sperm and DNA fragmentation index (DFI). Then the semen was diluted 1:19(semen: diluents v/ v) using a Tris diluents and subjected into slow cryopreservation program .The semen samples were assessed after freezing-thawing. Cryopreservation causes a clear decadency in some parameters of ram semen .Result shows that there was a significantly (p< 0.01) affect of cryopreservation on the dead sperms percentage (31.6 ± 1.92), individual motility(IM)(31.33± 1.20%) and DNA fragmentation index (DFI) (29.83± 4.45%).

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